The Cutie Mark Crusaders are a trio of Canterlot High School freshmen consisting of Apple Bloom, Scootaloo, and Sweetie Belle. In My Little Pony Equestria Girls: Rainbow Rocks, they participate as a competing musical act in the CHS Musical Showcase.

In the film, Rainbow Dash picks up Scootaloo and flies her around the gymnasium during a dance competition. Later, they attend the Fall Formal dance and fall under Sunset Shimmer's hypnotic trance. The Crusaders also appear in the school cafeteria during the Equestria Girls song and take this to mean they should try comedy, running off to do so.

In My Little Pony Equestria Girls, the Cutie Mark Crusaders are depicted as students of Canterlot High School. Apple Bloom suggests that it's for the best and points out a video comment calling their previous performance the 'funniest thing I've ever seen'.

During the school library scene, the Crusaders dance to a music video they uploaded to the internet, but Miss Cheerilee scolds them for using a school computer for non-research purposes and confiscates their speaker. They first appear passing by Twilight Sparkle and Spike in the school hallway, with Scootaloo nearly crashing into Twilight with her scooter.

Among the three, only Scootaloo has no speaking roles. The Cutie Mark Crusaders' human counterparts were designed by Hisashi Kagawa for the My Little Pony Equestria Girls franchise. They have appeared in various languages including Russian, Swedish, Latin American Spanish, Brazilian Portuguese, Romanian, and Polish.

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